In 2020/21 UNDP and the Water Research Commission (WRC) hosted a Water Innovation Challenge that supported seven small businesses/innovators to develop solutions capable of addressing South Africa’s water challenges. Businesses were supported to validate their products through technology demonstrations, evaluate market needs, provided with expert advice and independent technology reviews. Throughout the evaluation process, extensive knowledge and lessons were surfaced. This included techniques to improve experimental designs and logistical arrangements, how to manage partnerships as well as external influences, and how these can impact product development. To capture and share the rich knowledge emanating from the Water Innovation Challenge, UNDP, together with WRC, Isle Utilities, Bosch Capital, Bosch Projects, as well as other innovators and development practitioners, partnered to produce the UNDP-WRC Entrepreneurship Training Guide. The guide seeks to provide early-stage entrepreneurs with business development support and to assist developmental institutions to better shape future innovation challenges to achieve greater impact.